Description: Rabbit polyclonal antibody to HSC70 Immunogen: Recombinant protein corresponding to human HSC70. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: HSPA8 Alternative Names: HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1Entrez Gene (Human): 3312SwissProt (Human): P11142Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSC70 expression in Hela (A), HepG2 (B), Raw264.7 (C), mouse brain (D), rat brain (E), rat liver (F) whole cell lysates.
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Immunohistochemical analysis of HSC70 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.