Description: Rabbit polyclonal antibody to Histone H2B (Acetyl-K12) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K12 of human Histone H2B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: HIST1H2BC Alternative Names: HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/lEntrez Gene (Human): 255626; 3018; 3017; 8339; 8343; 8344; 8346; 8347Entrez Gene (Mouse) : 319177; 319178; 319179Entrez Gene (Rat) : 24829SwissProt (Human): Q96A08; P33778; P62807SwissProt (Mouse) : P70696; Q64475; Q6ZWY9SwissProt (Rat) : Q00729Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2B (Acetyl-K12) expression in Hela TSA-treated (A) whole cell lysates.
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  Immunohistochemical analysis of Histone H2B (Acetyl-K12) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.