Description: Rabbit polyclonal antibody to Histone H2A (Acetyl-K15) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/200 - 1/500) Gene Symbol: HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM; HIST2H2AA3; HIST2H2AA4; HIST3H2A Alternative Names: HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4Entrez Gene (Human): 8329; 8330; 8332; 8336; 8969; 723790; 8337; 92815Entrez Gene (Mouse) : 319164; 15267; 319162Entrez Gene (Rat) : 365877; 64646SwissProt (Human): P0C0S8; Q6FI13; Q7L7L0SwissProt (Mouse) : P22752; Q6GSS7; Q8BFU2SwissProt (Rat) : P02262; P0CC09; Q4FZT6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2A (Acetyl-K15) expression in Hela TSA-treated (A) whole cell lysates.
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  Immunohistochemical analysis of Histone H2A (Acetyl-K15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.