Description: Rabbit polyclonal antibody to Histone H1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200) Gene Symbol: HIST1H1A; HIST1H1C; HIST1H1D; HIST1H1E Alternative Names: HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4Entrez Gene (Human): 3024; 3006; 3007; 3008Entrez Gene (Mouse) : 80838; 50708; 14957; 50709SwissProt (Human): Q02539; P16403; P16402; P10412SwissProt (Mouse) : P43275; P15864; P43277; P43274Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H1 expression in Hela (A), NIH3T3 (B) whole cell lysates.
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  Immunohistochemical analysis of Histone H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.