Description: Rabbit polyclonal antibody to HIF1 beta Immunogen: Recombinant protein corresponding to human HIF1 beta. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: ARNT Alternative Names: BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-betaEntrez Gene (Human): 405SwissProt (Human): P27540Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HIF1 beta expression in MCF7 (A), NIH3T3 (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of HIF1 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.