Description: Rabbit polyclonal antibody to HIF1 alpha Immunogen: Recombinant protein corresponding to mouse HIF1 alpha. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: HIF1A Alternative Names: BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8Entrez Gene (Mouse) : 15251Entrez Gene (Rat) : 29560SwissProt (Mouse) : Q61221SwissProt (Rat) : O35800Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HIF1 alpha expression in mouse liver (A), rat liver (B) whole cell lysates.
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  Immunohistochemical analysis of HIF1 alpha staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.