Description: Rabbit polyclonal antibody to GAP43 Immunogen: Recombinant protein corresponding to human GAP43. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/3000), IH (1/500 - 1/1000) Gene Symbol: GAP43 Alternative Names: Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46Entrez Gene (Human): 2596Entrez Gene (Mouse) : 14432Entrez Gene (Rat) : 29423SwissProt (Human): P17677SwissProt (Mouse) : P06837SwissProt (Rat) : P07936Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GAP43 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of GAP43 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.