Description: Rabbit polyclonal antibody to Flotillin 2 Immunogen: Recombinant protein corresponding to human Flotillin 2. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: FLOT2 Alternative Names: ESA1; M17S1; Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1Entrez Gene (Human): 2319SwissProt (Human): Q14254Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Flotillin 2 expression in A431 (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of Flotillin 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.