Description: Rabbit polyclonal antibody to ERK5 Immunogen: Recombinant protein corresponding to human ERK5. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: MAPK7 Alternative Names: BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5Entrez Gene (Human): 5598SwissProt (Human): Q13164Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ERK5 expression in Hela (A), 293T (B), NIH3T3 (C), mouse skeletal muscle (D), rat kidney (E), rat skeletal muscle (F) whole cell lysates.
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Immunohistochemical analysis of ERK5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.