Description: Rabbit polyclonal antibody to Cyclin D1 Immunogen: Recombinant protein corresponding to mouse Cyclin D1. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CCND1 Alternative Names: BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogeneEntrez Gene (Mouse) : 12443Entrez Gene (Rat) : 58919SwissProt (Mouse) : P25322SwissProt (Rat) : P39948Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cyclin D1 expression in C2C12 (A), rat heart (B) whole cell lysates.
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  Immunohistochemical analysis of Cyclin D1 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.