Description: Rabbit polyclonal antibody to c-FOS Immunogen: Recombinant protein corresponding to human c-FOS. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: FOS Alternative Names: G0S7; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7Entrez Gene (Human): 2353Entrez Gene (Mouse) : 14281Entrez Gene (Rat) : 140675SwissProt (Human): P01100SwissProt (Mouse) : P01101SwissProt (Rat) : P12841Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of c-FOS expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of c-FOS staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.