Description: Rabbit polyclonal antibody to Calnexin Immunogen: Recombinant protein corresponding to human Calnexin. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: CANX Alternative Names: Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90Entrez Gene (Human): 821SwissProt (Human): P27824Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Calnexin expression in Hela (A), NIH3T3 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of Calnexin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.