Description: Rabbit polyclonal antibody to Calcium Sensing Receptor Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of mouse Calcium Sensing Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CASR Alternative Names: GPRC2A; PCAR1; Extracellular calcium-sensing receptor; CaSR; Parathyroid cell calcium-sensing receptor 1; PCaR1Entrez Gene (Mouse) : 12374Entrez Gene (Rat) : 24247SwissProt (Mouse) : Q9QY96SwissProt (Rat) : P48442Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Calcium Sensing Receptor expression in mouse brain (A), rat brain (B) whole cell lysates.
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Immunohistochemical analysis of Calcium Sensing Receptor staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.