Description: Rabbit polyclonal antibody to Beta2A-tubulin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta2A-tubulin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/2000 - 1/5000), IH (1/200 - 1/500) Gene Symbol: TUBB2A Alternative Names: TUBB2; Tubulin beta-2A chain; Tubulin beta class IIaEntrez Gene (Human): 7280Entrez Gene (Mouse) : 22151Entrez Gene (Rat) : 498736SwissProt (Human): Q13885SwissProt (Mouse) : Q7TMM9SwissProt (Rat) : P85108Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta2A-tubulin expression in Hela (A), NIH3T3 (B), rat kidney (C) whole cell lysates.
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Immunohistochemical analysis of Beta2A-tubulin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.