Description: Rabbit polyclonal antibody to ACTN1 Immunogen: Recombinant protein corresponding to mouse ACTN1. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: ACTN1 Alternative Names: Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1Entrez Gene (Mouse) : 109711Entrez Gene (Rat) : 81634SwissProt (Mouse) : Q7TPR4SwissProt (Rat) : Q9Z1P2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ACTN1 expression in NIH3T3 (A), PC12 (B), rat heart (C) whole cell lysates.
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  Immunohistochemical analysis of ACTN1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.