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Anti-14-3-3 epsilon Antibody CPA9222
  • Western blot analysis of 14-3-3 epsilon expression in mouse brain (A), rat brain (B) whole cell lysates.
  • Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-14-3-3 epsilon Antibody
Cat No: CPA9222
Source: Rabbit
Reactivity: M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to 14-3-3 epsilon
Immunogen: Recombinant protein corresponding to mouse 14-3-3 epsilon.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500)
Gene Symbol: YWHAE
Alternative Names: 14-3-3 protein epsilon; 14-3-3E
Entrez Gene (Mouse) : 22627
Entrez Gene (Rat) : 29753
SwissProt (Mouse) : P62259
SwissProt (Rat) : P62260
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of 14-3-3 epsilon expression in mouse brain (A), rat brain (B) whole cell lysates.
  • Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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