Description: Rabbit polyclonal antibody to 14-3-3 epsilon Immunogen: Recombinant protein corresponding to mouse 14-3-3 epsilon. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: YWHAE Alternative Names: 14-3-3 protein epsilon; 14-3-3EEntrez Gene (Mouse) : 22627Entrez Gene (Rat) : 29753SwissProt (Mouse) : P62259SwissProt (Rat) : P62260Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of 14-3-3 epsilon expression in mouse brain (A), rat brain (B) whole cell lysates.
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Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.