Description: Mouse monoclonal antibody to TNF alpha Immunogen: Recombinant protein corresponding to human TNF alpha. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TNF Alternative Names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aEntrez Gene (Human): 7124Entrez Gene (Mouse) : 21926Entrez Gene (Rat) : 24835SwissProt (Human): P01375SwissProt (Mouse) : P06804SwissProt (Rat) : P16599Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates.
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  Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.