Description: Mouse monoclonal antibody to Swiprosin-2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Swiprosin-2. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IF/IC (1/100 - 1/200) Gene Symbol: EFHD1 Alternative Names: SWS2; EF-hand domain-containing protein D1; EF-hand domain-containing protein 1; Swiprosin-2Entrez Gene (Human): 80303Entrez Gene (Mouse) : 98363SwissProt (Human): Q9BUP0SwissProt (Mouse) : Q9D4J1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates.
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  Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).