Description: Mouse monoclonal antibody to Survivin Immunogen: Recombinant protein corresponding to human Survivin. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: BIRC5 Alternative Names: API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivinEntrez Gene (Human): 332Entrez Gene (Mouse) : 11799Entrez Gene (Rat) : 64041SwissProt (Human): O15392SwissProt (Mouse) : O70201SwissProt (Rat) : Q9JHY7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Survivin expression in Hela (A), 293 (B), PC12 (C) whole cell lysates.
• 
  Immunohistochemical analysis of Survivin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.