Description: Mouse monoclonal antibody to Peroxiredoxin 1 Immunogen: Recombinant protein corresponding to human Peroxiredoxin 1. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200) Gene Symbol: PRDX1 Alternative Names: PAGA; PAGB; TDPX2; Peroxiredoxin-1; Natural killer cell-enhancing factor A; NKEF-A; Proliferation-associated gene protein; PAG; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2Entrez Gene (Human): 5052Entrez Gene (Mouse) : 18477Entrez Gene (Rat) : 117254SwissProt (Human): Q06830SwissProt (Mouse) : P35700SwissProt (Rat) : Q63716Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Peroxiredoxin 1 expression in MCF7 (A), mouse brain (B), rat kidney (C) whole cell lysates.
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Immunofluorescent analysis of Peroxiredoxin 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).