Description: Mouse monoclonal antibody to HSP90 beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human HSP90 beta. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/3000), IH (1/200 - 1/500) Gene Symbol: HSP90AB1 Alternative Names: HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84Entrez Gene (Human): 3326Entrez Gene (Mouse) : 15516Entrez Gene (Rat) : 301252SwissProt (Human): P08238SwissProt (Mouse) : P11499SwissProt (Rat) : P34058Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSP90 beta expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of HSP90 beta staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.