Description: Mouse monoclonal antibody to Histone H2B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/3000), IH (1/200 - 1/500) Gene Symbol: HIST1H2BB Alternative Names: H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/fEntrez Gene (Human): 3018Entrez Gene (Mouse) : 319178SwissProt (Human): P33778SwissProt (Mouse) : Q64475Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates.
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  Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.