Description: Mouse monoclonal antibody to Gamma-enolase Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Gamma-enolase. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200) Gene Symbol: ENO2 Alternative Names: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSEEntrez Gene (Human): 2026Entrez Gene (Mouse) : 13807Entrez Gene (Rat) : 100911625SwissProt (Human): P09104SwissProt (Mouse) : P17183SwissProt (Rat) : P07323Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Gamma-enolase expression in Hela (A), Jurkat (B), 293T (C) whole cell lysates.
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Immunohistochemical analysis of Gamma-enolase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.