Description: Mouse monoclonal antibody to Cytokeratin 8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 8. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/2000 - 1/5000), IH (1/100 - 1/200) Gene Symbol: KRT8 Alternative Names: CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8Entrez Gene (Human): 3856Entrez Gene (Mouse) : 16691Entrez Gene (Rat) : 25626SwissProt (Human): P05787SwissProt (Mouse) : P11679SwissProt (Rat) : Q10758Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytokeratin 8 expression in Hela (A), mouse brain (B) whole cell lysates.
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  Immunohistochemical analysis of Cytokeratin 8 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.