Description: Mouse monoclonal antibody to Cytokeratin 7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 7. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200), IP (1/50 - 1/200) Gene Symbol: KRT7 Alternative Names: SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7Entrez Gene (Human): 3855Entrez Gene (Mouse) : 110310Entrez Gene (Rat) : 300242SwissProt (Human): P08729SwissProt (Mouse) : Q9DCV7SwissProt (Rat) : Q6IG12Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Cytokeratin 7 expression in Hela (A), mouse kidney (B), mouse brain (C) whole cell lysates.
-
Immunohistochemical analysis of Cytokeratin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Immunoprecipitation of Cytokeratin 7 from 0.5mg Hela whole cell extract lysate, using Anti-Cytokeratin 7 Antibody.