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Anti-Cytokeratin 7 Antibody CPA9151
  • Western blot analysis of Cytokeratin 7 expression in Hela (A), mouse kidney (B), mouse brain (C) whole cell lysates.
  • Immunohistochemical analysis of Cytokeratin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of Cytokeratin 7 from 0.5mg Hela whole cell extract lysate, using Anti-Cytokeratin 7 Antibody.
Product NameAnti-Cytokeratin 7 Antibody
Cat No: CPA9151
Source: Mouse
Reactivity: H, M, R
Applications: WB, IH, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Mouse monoclonal antibody to Cytokeratin 7
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 7. The exact sequence is proprietary.
Clonality: Monoclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200), IP (1/50 - 1/200)
Gene Symbol: KRT7
Alternative Names: SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Entrez Gene (Human): 3855
Entrez Gene (Mouse) : 110310
Entrez Gene (Rat) : 300242
SwissProt (Human): P08729
SwissProt (Mouse) : Q9DCV7
SwissProt (Rat) : Q6IG12
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Cytokeratin 7 expression in Hela (A), mouse kidney (B), mouse brain (C) whole cell lysates.
  • Immunohistochemical analysis of Cytokeratin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of Cytokeratin 7 from 0.5mg Hela whole cell extract lysate, using Anti-Cytokeratin 7 Antibody.
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