Description: Mouse monoclonal antibody to Beta-tubulin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/3000 - 1/10000), IH (1/50 - 1/100), IP (1/50 - 1/100) Gene Symbol: TUBB3 Alternative Names: TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-IIIEntrez Gene (Human): 10381Entrez Gene (Mouse) : 22152Entrez Gene (Rat) : 246118SwissProt (Human): Q13509SwissProt (Mouse) : Q9ERD7SwissProt (Rat) : Q4QRB4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.
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Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using Anti-Beta-tubulin Antibody.