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Anti-Beta-tubulin Antibody CPA9128
  • Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.
  • Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using Anti-Beta-tubulin Antibody.
Product NameAnti-Beta-tubulin Antibody
Cat No: CPA9128
Source: Mouse
Reactivity: H, M, R, Mk, D, C, Rb
Applications: WB, IH, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$130
100 μl
$80
30 μl
$40
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Description: Mouse monoclonal antibody to Beta-tubulin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.
Clonality: Monoclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/3000 - 1/10000), IH (1/50 - 1/100), IP (1/50 - 1/100)
Gene Symbol: TUBB3
Alternative Names: TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Entrez Gene (Human): 10381
Entrez Gene (Mouse) : 22152
Entrez Gene (Rat) : 246118
SwissProt (Human): Q13509
SwissProt (Mouse) : Q9ERD7
SwissProt (Rat) : Q4QRB4
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.
  • Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using Anti-Beta-tubulin Antibody.
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