Description: Mouse monoclonal antibody to Beta-tubulin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/5000 - 1/10000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200) Gene Symbol: TUBB3 Alternative Names: TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-IIIEntrez Gene (Human): 10381Entrez Gene (Mouse) : 22152Entrez Gene (Rat) : 246118SwissProt (Human): Q13509SwissProt (Mouse) : Q9ERD7SwissProt (Rat) : Q4QRB4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Beta-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of Beta-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Beta-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).