Description: Mouse monoclonal antibody to Beta-catenin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-catenin. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200) Gene Symbol: CTNNB1 Alternative Names: CTNNB; Catenin beta-1; Beta-cateninEntrez Gene (Human): 1499Entrez Gene (Mouse) : 12387Entrez Gene (Rat) : 84353SwissProt (Human): P35222SwissProt (Mouse) : Q02248SwissProt (Rat) : Q9WU82Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Beta-catenin expression in Hela (A), 293T (B), MCF7 (C), mouse brain (D), rat liver (E) whole cell lysates.
-
Immunohistochemical analysis of Beta-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.