Description: Mouse monoclonal antibody to Beta1-tubulin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta1-tubulin. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/5000 - 1/10000), IH (1/200 - 1/500) Gene Symbol: TUBB1 Alternative Names: Tubulin beta-1 chainEntrez Gene (Human): 81027Entrez Gene (Mouse) : 545486SwissProt (Human): Q9H4B7SwissProt (Mouse) : A2AQ07Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta1-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of Beta1-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.