Description: Mouse monoclonal antibody to AMACR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human AMACR. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200) Gene Symbol: AMACR Alternative Names: Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemaseEntrez Gene (Human): 23600Entrez Gene (Mouse) : 17117SwissProt (Human): Q9UHK6SwissProt (Mouse) : O09174SwissProt (Rat) : P70473Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AMACR expression in HepG2 (A), mouse kidney (B) whole cell lysates.
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Immunohistochemical analysis of AMACR staining in mouse prostate adenocancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.