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Anti-Alpha-tubulin Antibody CPA9108
  • Western blot analysis of Alpha-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
  • Immunohistochemical analysis of Alpha-tubulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Alpha-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Immunoprecipitation of Alpha-tubulin from 0.5mg mouse brain whole cell extract lysate, using Anti-Alpha-tubulin Antibody.
Product NameAnti-Alpha-tubulin Antibody
Cat No: CPA9108
Source: Mouse
Reactivity: H, M, R
Applications: WB, IH, IF/IC, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$130
100 μl
$80
30 μl
$40
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Description: Mouse monoclonal antibody to Alpha-tubulin
Immunogen: Recombinant protein corresponding to human Alpha-tubulin.
Clonality: Monoclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/3000 - 1/10000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/50 - 1/100)
Gene Symbol: TUBA1A
Alternative Names: TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain
Entrez Gene (Human): 7846; 10376
Entrez Gene (Mouse) : 22142; 22143
Entrez Gene (Rat) : 64158; 500929
SwissProt (Human): Q71U36; P68363
SwissProt (Mouse) : P68369; P05213
SwissProt (Rat) : P68370; Q6P9V9
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Alpha-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
  • Immunohistochemical analysis of Alpha-tubulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Alpha-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Immunoprecipitation of Alpha-tubulin from 0.5mg mouse brain whole cell extract lysate, using Anti-Alpha-tubulin Antibody.
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