Description: Mouse monoclonal antibody to Alpha-tubulin (Acetyl-K40) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K40 of human Alpha-tubulin protein. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/100 - 1/200) Gene Symbol: TUBA1B Alternative Names: Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chainEntrez Gene (Human): 7277SwissProt (Human): P68363Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Alpha-tubulin (Acetyl-K40) expression in HeLa (A) whole cell lysates.
-
Immunohistochemical analysis of Alpha-tubulin (Acetyl-K40) staining in human breast cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.