Anti-Beta-actin Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), rat brain (E), COS7 (F) whole cell lysates.
Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Beta-actin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name	Anti-Beta-actin Antibody
Cat No:	CPA9100
Source:	Mouse
Reactivity:	H, M, R, C, D, Mk, Rb, Insect
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$130

100 μl

$80

30 μl

$40

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Description: Mouse monoclonal antibody to Beta-actin

Immunogen: Recombinant protein corresponding to human Beta-actin.

Clonality: Monoclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/3000 - 1/10000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)

Gene Symbol: ACTB

Alternative Names: Actin cytoplasmic 1; Beta-actin

Entrez Gene (Human): 60

Entrez Gene (Mouse) : 11461

Entrez Gene (Rat) : 81822

SwissProt (Human): P60709

SwissProt (Mouse) : P60710

SwissProt (Rat) : P60711

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), rat brain (E), COS7 (F) whole cell lysates.
Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Beta-actin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Application WB

Reactivity Mouse

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