Anti-GAPDH Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of GAPDH expression in Hela (A), mouse brain (B), mouse spleen (C), rat liver (D), rat lung (E) whole cell lysates.
Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GAPDH staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Name	Anti-GAPDH Antibody
Cat No:	CPA9067
Source:	Mouse
Reactivity:	H, M, R
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$130

100 μl

$80

30 μl

$40

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Description: Mouse monoclonal antibody to GAPDH

Immunogen: Recombinant protein corresponding to GAPDH.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Monoclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/3000 - 1/10000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)

Gene Symbol: GAPDH

Alternative Names: GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Entrez Gene (Human): 2597

Entrez Gene (Mouse) : 100042025

Entrez Gene (Rat) : 24383

SwissProt (Human): P04406

SwissProt (Mouse) : P16858

SwissProt (Rat) : P04797

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of GAPDH expression in Hela (A), mouse brain (B), mouse spleen (C), rat liver (D), rat lung (E) whole cell lysates.
Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GAPDH staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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