Description: Mouse monoclonal antibody to V5-tag Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of V5-tag. The exact sequence is proprietary. Clonality: Monoclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200) Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of over-expressed V5-tagged protein in 293T cell lysate.
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Immunofluorescent analysis of V5-tag staining in 293T cells transfected with a V5-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Immunoprecipitation of V5-tagged protein from HEK293T cells transfected with vector overexpressing V5 tag, using Anti-V5-tag Antibody.