Description: Rabbit polyclonal antibody to NFAT5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFAT5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NFAT5 Alternative Names: KIAA0827; TONEBP; Nuclear factor of activated T-cells 5; NF-AT5; T-cell transcription factor NFAT5; Tonicity-responsive enhancer-binding protein; TonE-binding protein; TonEBPEntrez Gene (Human): 10725SwissProt (Human): O94916Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NFAT5 expression in Hela (A), HEK293T (B) whole cell lysates.
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  Immunohistochemical analysis of NFAT5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.