Description: Rabbit polyclonal antibody to SPFH1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPFH1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ERLIN1; ERLIN2 Alternative Names: ERLIN1; C10orf69; KE04; KEO4; SPFH1; Erlin-1; Endoplasmic reticulum lipid raft-associated protein 1; Protein KE04; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1; SPFH domain-containing protein 1; ERLIN2; C8orf2; SPFH2; Erlin-2; Endoplasmic reticulum lipid raft-associated protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2; SPFH domain-containing protein 2Entrez Gene (Human): 10613; 11160Entrez Gene (Mouse) : 226144; 244373Entrez Gene (Rat) : 290823SwissProt (Human): O75477; O94905SwissProt (Mouse) : Q91X78; Q8BFZ9SwissProt (Rat) : B5DEH2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of SPFH1 expression in HepG2 (A), NIH3T3 (B), Hela (C) whole cell lysates.
-
Immunohistochemical analysis of SPFH1 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.