Description: Rabbit polyclonal antibody to PIR121 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PIR121. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CYFIP2 Alternative Names: KIAA1168; PIR121; Cytoplasmic FMR1-interacting protein 2; p53-inducible protein 121Entrez Gene (Human): 26999Entrez Gene (Mouse) : 76884SwissProt (Human): Q96F07SwissProt (Mouse) : Q5SQX6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PIR121 expression in HepG2 (A), Hela (B) whole cell lysates.
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Immunohistochemical analysis of PIR121 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.