Description: Rabbit polyclonal antibody to Tyrosyl-tRNA Synthetase Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Tyrosyl-tRNA Synthetase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: YARS Alternative Names: Tyrosine--tRNA ligase, cytoplasmic; Tyrosyl-tRNA synthetase; TyrRSEntrez Gene (Human): 8565Entrez Gene (Mouse) : 107271Entrez Gene (Rat) : 313047SwissProt (Human): P54577SwissProt (Mouse) : Q91WQ3SwissProt (Rat) : Q4KM49Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Tyrosyl-tRNA Synthetase expression in Hela (A), U87MG (B) whole cell lysates.
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  Immunohistochemical analysis of Tyrosyl-tRNA Synthetase staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.