Description: Rabbit polyclonal antibody to MIB1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MIB1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MIB1 Alternative Names: DIP1; KIAA1323; ZZANK2; E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1; Zinc finger ZZ type with ankyrin repeat domain protein 2Entrez Gene (Human): 57534Entrez Gene (Mouse) : 225164SwissProt (Human): Q86YT6SwissProt (Mouse) : Q80SY4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MIB1 expression in HEK293T (A), K562 (B), Hela (C) whole cell lysates.
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  Immunohistochemical analysis of MIB1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.