Description: Rabbit polyclonal antibody to CK1 alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CK1 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CSNK1A1 Alternative Names: Casein kinase I isoform alpha; CKI-alpha; CK1Entrez Gene (Human): 1452Entrez Gene (Mouse) : 93687Entrez Gene (Rat) : 113927SwissProt (Human): P48729SwissProt (Mouse) : Q8BK63SwissProt (Rat) : P97633Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CK1 alpha expression in Hela (A), K562 (B) whole cell lysates.
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  Immunohistochemical analysis of CK1 alpha staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.