Description: Rabbit polyclonal antibody to PTEN Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PTEN Alternative Names: MMAC1; TEP1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homologEntrez Gene (Human): 5728Entrez Gene (Mouse) : 19211SwissProt (Human): P60484SwissProt (Mouse) : O08586Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PTEN expression in Hela (A), KB (B), mouse heart (C), mouse brain (D), mouse lung (E) whole cell lysates.
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  Immunohistochemical analysis of PTEN staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.