Description: Rabbit polyclonal antibody to Ub Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ub. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: UBA52; RPS27A; UBB; UBC Alternative Names: UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1Entrez Gene (Human): 7311; 7314; 7316; 6233Entrez Gene (Mouse) : 22187; 22190; 78294; 22186Entrez Gene (Rat) : 192255; 50522; 100912032; 64156SwissProt (Human): P0CG47; P0CG48; P62979; P62987Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ub expression in K562 (A), L929 (B) whole cell lysates.
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  Immunohistochemical analysis of Ub staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.