Description: Rabbit polyclonal antibody to EGFR (Phospho-Y1197) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1197 of human EGFR protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EGFR Alternative Names: ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1Entrez Gene (Human): 1956Entrez Gene (Mouse) : 13649SwissProt (Human): P00533SwissProt (Mouse) : Q01279Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EGFR (Phospho-Y1197) expression in A431 (A), NIH3T3 (B) whole cell lysates.
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Immunohistochemical analysis of EGFR (Phospho-Y1197) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.