Description: Rabbit polyclonal antibody to ATM Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATM. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ATM Alternative Names: Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutatedEntrez Gene (Human): 472Entrez Gene (Mouse) : 11920SwissProt (Human): Q13315SwissProt (Mouse) : Q62388Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATM expression in K562 (A), mouse brain (B) whole cell lysates.
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  Immunohistochemical analysis of ATM staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.