Description: Rabbit polyclonal antibody to CD235a Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD235a. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: GYPA Alternative Names: GPA; Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD235aEntrez Gene (Human): 2993SwissProt (Human): P02724Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD235a expression in HEK293T (A), 22RV1 (B) whole cell lysates.
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Immunohistochemical analysis of CD235a staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.