Description: Rabbit polyclonal antibody to CD158e Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD158e. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: KIR3DL1 Alternative Names: CD158E; NKAT3; NKB1; Killer cell immunoglobulin-like receptor 3DL1; CD158 antigen-like family member E; HLA-BW4-specific inhibitory NK cell receptor; MHC class I NK cell receptor; Natural killer-associated transcript 3; NKAT-3; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11; CD158eEntrez Gene (Human): 3811SwissProt (Human): P43629SwissProt (Mouse) : P83555Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD158e expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of CD158e staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.