Description: Rabbit polyclonal antibody to CD19 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD19. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CD19 Alternative Names: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19Entrez Gene (Human): 930Entrez Gene (Mouse) : 12478SwissProt (Human): P15391SwissProt (Mouse) : P25918Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of CD19 expression in mouse spleen (A), rat spleen (B), SGC7901 (C), HCT116 (D) whole cell lysates.
• 
  Immunohistochemical analysis of CD19 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.