Description: Rabbit polyclonal antibody to CALB1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CALB1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CALB1 Alternative Names: CAB27; Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-typeEntrez Gene (Human): 793Entrez Gene (Rat) : 83839SwissProt (Human): P05937SwissProt (Rat) : P07171Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CALB1 expression in K562 (A), rat kidney (B) whole cell lysates.
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  Immunohistochemical analysis of CALB1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.