Description: Rabbit polyclonal antibody to CD133 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD133. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PROM1 Alternative Names: PROML1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133Entrez Gene (Human): 8842Entrez Gene (Mouse) : 19126SwissProt (Human): O43490SwissProt (Mouse) : O54990Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD133 expression in K562 (A), SW620 (B), Jurkat (C), mouse kidney (D), mouse lung (E) whole cell lysates.
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  Immunohistochemical analysis of CD133 staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.